Sort By Filter By. Restriction Digest: Restriction Digest cleaves a DNA sequence in a virtual restriction digest, with one, two, or three restriction enzymes. This data was culled from suppliers catalogues (mainly New England Biolabs and Roche Molecular Biochemicals). KasI. Restriction enzymes were obtained from the following suppliers and stored at −20°C: BbeI, Takara Biomedicals, Japan; EgeI and Mly113I, SibEnzyme, Russia; EheI, MBI Fermentas, Lithuania; KasI, NarI and SfoI, New England Biolabs, USA. Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV). On pBR322, the NarI site at 548 bp is cut very slowly. R0544L. Brand: New England Biolabs. The diversity of reaction mechanisms employed by Type II restriction enzymes was investigated by ana-lysing the reactions of seven endonucleases at the same DNA sequence. 5'– GCC ↓ GGC –3' 3'– CGG ↑ CCG –5' NarI EN-E2291 Isoschizomers: Mly113I, Neoschizomers: BbeI, DinI, EgeI, EheI, KasI, SfoI . Locating restriction enzyme cutting sites. The first letter of the genus name is capitalized and followed by the first two letters of the species name (consequently, these three letters are in italics). Underlined restriction enzymes are neoschizomer (recognize same sequence but … Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI). Restriction enzymes have names derived from the initials of the bacteria they come from. Enzymes that are part of the restriction-modification systems. KasI RM 380.00 – RM 1,465.00. KasI, recombinant. From OpenWetWare. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Category: ... Name: KasI: Cutsite: G ↓ GCGC ↑ C: Overhang: 5′ GCGC: Name: KpnI: Cutsite: G ↑ GTAC ↓ C: Overhang: 3′ GTAC: Name: KpnI-HF® Cutsite: G ↑ GTAC ↓ C: Overhang: 3′ GTAC: Name: LpnPI: Cutsite: C m CDG N₁₀ ↓ NNNN ↑ Overhang: 5′ NNNN: Name: MboI: Cutsite: � NarI, KasI, Mly113I, SfoI, EgeI, EheI and BbeI cleave DNA at several different posi-tions in the sequence 50-GGCGCC-30. Code; Contact; Automate RestrictionMapper; Other Free Molecular Biology Resources; Dilution Calculator; Conformation. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in … Their concentrations are noted in terms of units of enzyme activity per millilitre, as specified by the supplier. This is an interface to a utility that determines the locations at which the selected enzyme is cutting the human sequence. The strain is sometimes represented (e.g., the R in EcoRI refers to E. coli strain RY13). Back to Sauer:Enzymes. Dec 14, 2015 - CLUE: Lesson 1.4 An example of a restriction enzyme. All available prototype enzymes are marked green, yellow marks indicate the availability of an isoschizomer * with identical recognition sequence and cutting pattern only isolated from a different strain of bacteria. NaeI EN-122 Isoschizomers: PdiI, Neoschizomers: MroNI, NgoMIV. 5'– GG ↓ CG CC –3' 3'– CC GC ↑ GG –5' NcoI EN-123 Isoschizomers: Bsp19I JBSpeed Restriction Enzyme. KasI, recombinant. Minimum Site Length. Under standard restriction digest conditions NarI requires two sites for efficient digestion. KasI MluI NarI NdeI PacI PflMI PmlI PpuMI Psp5II PspAI RsrII SgfI SgrAI SmaI SnaBI SplI SrfI Sse8387I SwaI VspI XcmI XmaI Restriction Enzymes That Do Not Cut the pGL4.78[hRlucCP/Hygro] Vector. Maximum Cuts. Home Protocols Lab Members Materials Equipment Links Internal NOTE: Some enzymes listed may be very old and you may need to order a new tube. The resulting fragments are sorted by size, and they are given a title specifying their length, their position in the original sequence, and the enzyme sites that produced them. It contains approximately 90 enzymes. Products & Ordering. genomic DNA was digested with AflIII, AgeI, AseI, BglII, BsaHI, BsrFI, BstYI, ClaI, EcoRI, KasI, KpnI, MluI, NgoMI, PaeR7I, Ppu10I, or PstI restriction enzymes or any other restriction enzymes that will give rise to reasonable size template DNA (less than 10 kb) for inverse PCR reaction. Restriction Enzymes Enzyme Finder: The Enzyme Finder - Flyer leads you to the appropriate Jena Bioscience enzyme. If so specified, alternative enzymes that cut the sequence at the same location are reported. NarI, KasI, Mly113I, SfoI, EgeI, EheI and BbeI cleave DNA at several different positions in the sequence 5′-GGCGCC-3′. RM 379.00 – RM 1,443.00. Check in the freezer first. Restriction endonucleases are enzymes which cleave double-stranded DNA in a site- specific manner. Restriction enzymes: commercial source All endonucleases bellow are isoschizomers Recognition sequence : R'AATT_Y. Restriction Enzymes That Cut the pGL4.10[luc2] Vector Between 1 and 5 Times. .. ® The supporting documents available for this product can be downloaded below. Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. XbaI cuts twice in damŒ strains. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. 250 units-1 + Unavailable in your region . Antibodies & Protein Biology Antibody Production & Purification; Electrophoresis, Western Blotting and ELISA This document lists available enzymes alphabetically by enzyme name, and by cleavage site. discovering new restriction enzyme specifi cities. Circular Linear . Maps sites for restriction enzymes, a.k.a. Protocol Double Digest Protocol with Standard Restriction Enzymes. A vial of 6X Purple Load Dye is included with most restriction enzymes. … Type II restriction enzymes was investigated by ana-lysing the reactions of seven endonucleases at the same DNA sequence. Brand: New England Biolabs. NarI produces a 2-base 5´ extension whereas KasI produces a 4-base 5´ extension. SAUER LAB. Fermentas is the supplier of choice both for classic restriction enzymes and for new unique enzymes, which are not supplied by other companies. Demonstrates marked site preferences. KasI restriction enzyme wherein the enzyme first binds and cleaves at palindromic sequence at one strand, gets detached, then again binds to the same site and cleaves second strand at a much slower rate (25 times lower)21. Note: XbaI cuts the vector once in dam+ strains, as a second XbaI site downstream of the selectable marker is protected by methylation. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Recognition sequence, reaction conditions, heat denaturation, and microbial source for KasI restriction enzyme. Over 210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. This article contains a list of the most studied restriction enzymes whose names start with G to K inclusive. Enzyme No. Ca -Dz Restriction Enzymes Thermo Scientific™ DpnI (10 U/µL) Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). Enzyme Location Notes AatI enzyme freezer Acc65I enzyme freezer AccI enzyme freezer AflII enzyme freezer … Select Individual Enzymes. Also does virtual digestion. SfoI (BbeI, KasI, NarI) 5=-GGC^GCC-3= 27 0.12 ND New England BioLabs SpeI (AhlI, BcuI) 5=-A^CTAGT-3= 31 0.126 0.126 New England BioLabs SbfI (SdaI, Sse8387I) 5=-CCTGCA^GG-3= 28 0.134 10.72 New England BioLabs PasI 5=-CC^CWGGG-3= 24 ND ND ND a Restriction enzymes in parentheses recognize the same sequence. KasI KpnI MluI NarI NheI PacI PaeR7I PflMI PinAI PmeI PmlI PpuMI Psp5II PspAI PvuII RsrII SgfI SgrAI SmaI SphI SplI SrfI Sse8387I StuI SwaI XcmI XhoI XmaI Restriction Enzymes That Do Not Cut the pGL4.75[hRluc/CMV] Vector. Restriction Enzymes: "N" Enzymes. Different Types of restriction enzymes. Saccharopolyspora sp. Include. 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